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Free · Unbranded · Compliance & logs

Veterinary Autoclave Sterilization Log
Free PDF.

A log for every autoclave cycle: what was in the load, the temperature, pressure, and time reached, whether the chemical indicators changed, and the result of each biological indicator test. If a pack is ever questioned, the cycle can be traced.

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  • 1 page
  • Landscape
  • Indicator results
  • Traceable loads

Unbranded: no logo or linksNo account neededPrint at 100% on US LetterUpdated November 10, 2026

Preview of page one of the Autoclave Sterilization Log
PDF · 1 page

What’s on the form

Every section, and what it’s for.

01

Sterilizer details

Unit, model, serial number, and the month the sheet covers.

02

Cycle log

Eighteen cycles with date, cycle number, load contents, cycle type, temperature, pressure, time, indicator results, and operator.

03

Biological indicators

Spore test lot, the date and result, and the control result.

04

Failed cycles

What to record and do when an indicator fails or a cycle aborts.

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Type it, then print or save.

Every line on the form is editable right here. When you’re done, print it or choose “Save as PDF” in the print dialog. Everything stays in your browser.

Click any line to type, or add your logo. Nothing is uploaded or saved.

Full size

Using it well

Small habits that make it worth the paper.

Number every cycle

Write the cycle number on each pack label. It is the link between a pack on the shelf and this log.

Run spore tests on a schedule

Many practices run a biological indicator weekly and with every load containing implants. Follow your manufacturer’s instructions.

Quarantine after a failure

If an indicator fails, pull the packs from that cycle, reprocess them, and record what you did.

Questions about
this form.

Printing, editing, and what the form does and doesn’t cover.

What is the difference between chemical and biological indicators?+

Chemical indicators change color when exposed to certain conditions, such as temperature and steam, and are used on or in every pack. Biological indicators contain bacterial spores and show whether conditions were enough to kill them; they are the most direct test that sterilization worked.

How often should a biological indicator be run?+

Commonly at least weekly, and with any load containing implants, but follow your sterilizer manufacturer’s instructions and any state or accreditation requirements.

What temperature does an autoclave reach?+

Steam sterilizers commonly run at about 121 °C (250 °F) or 132 to 134 °C (270 to 273 °F), with exposure times that depend on the cycle and load. Use the settings in your manufacturer’s manual.

What should happen after a failed cycle?+

Do not use the load. Reprocess it, check the loading and the machine, and if a biological indicator failed, consider whether earlier loads need to be recalled. Record each step.

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A starting point, not legal or clinical advice. VetStack’s forms are general templates. Requirements for medical records, consent, and controlled substances vary by state and practice, so review the wording with your practice’s attorney and check your state veterinary board’s rules before adopting a form as standard.

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